Journal: Experimental and Therapeutic Medicine
Article Title: Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation
doi: 10.3892/etm.2020.9060
Figure Lengend Snippet: Analysis of the parameters of frozen sperm. (A) Microscopy images. (a) The first row presents diff Quik sperm staining (The white box marks two sperm with obvious abnormalities in the neck and the red arrow is used as the marker); (b) The second row displays eosin aniline black smear under the light field microscope (scale bar, 20 µm). (B) Quantified parameters of the sperm. (a) Sperm survival rate in each group; (b) sperm recovery rate after freezing; (c) sperm morphology analysis; (d) sperm abnormal morphology classification analysis. (n=22). # P<0.05 vs. GEYC group. GEYC, glycerin-yolk-citrate cryopreservative; LBP, Lycium barbarum polysaccharides; D, dead sperms were indicated by red or dark pink staining of the head; L, live sperms were indicated a white or light pink head.
Article Snippet: The sperm morphology was observed using a diff Quik rapid staining kit (cat. no. G1541; Beijing Solarbio Science & Technology Co., Ltd.) First, the sperm concentration was adjusted to (2-50x10 6 ) using normal saline, and a sperm smear was then prepared and immersed in fast dye solution I at room temperature for 10 sec, followed by fast dye solution II at room temperature for 5 sec.
Techniques: Microscopy, Diff-Quik, Staining, Marker